Journal: BMC Ophthalmology

Article Title: A comparison of clinical outcomes and optical performance between monofocal and new monofocal with enhanced intermediate function intraocular lenses: a case-control study

doi: 10.1186/s12886-021-02124-w

Figure Lengend Snippet: Schematic diagram of the optical bench system used in this study (LED = light emitting diode; USAF = United States Air Force; IOL = intraocular lens; CMOS = complementary metal–oxide–semiconductor)

Article Snippet: The optical bench system used in this study consisted of a LED light, the 1951 United States Air Force (1951 USAF) resolution test chart, an artificial pupil, a pupil camera, trial lens, model eye, and complementary metal-oxide-semiconductor (CMOS) camera (BFS-U3-120S4M-CS; FLIR Systems Inc., Wilsonville, OR) (Fig. ) [ ].

Techniques:

Journal: Science advances

Article Title: Shaping early neural development by timed elevated tissue oxygen tension: Insights from multiomic analysis on human cerebral organoids.

doi: 10.1126/sciadv.ado1164

Figure Lengend Snippet: Fig. 2. Intra-organoid oxygen tension, structure, and cell compositions of hCOs. (A) Bright-field images of the control hCOs cultured in the hydrogel matrix at different time points (scale bar, 400 μm), and fluorescence images of the control hCOs stained with a plasma membrane stain, CellMask (green), and the embedded oxygen-sensing CPOx beads (red) (scale bar, 400 μm). The representative CPOx beads embedded within the hCOs are denoted by white line squares. (B) Organoid sizes of the hCOs esti- mated from the captured bright-field images. Data are presented as box plots with all data points and mean (SD) (n = 10). (C) Quantitative results of the average oxygen tension values calculated based on the fluorescence lifetime measurement of CPOx beads embedded within the hCOs (intra-organoid oxygen tension) or within the hy- drogel but not embedded within the hCOs (hydrogel oxygen tension). The data are expressed as mean (SD). The quantitative data are shown in table S2. (D) Confocal images of hCO slices stained with TUBB3, SOX2, Nestin, and Ki-67 proteins and cell nuclei [4′,6-diamidino-2-phenylindole (DAPI)] reveal the distribution of neurons and NSCs in the hCOs at weeks 4 to 6 (scale bar, 400 μm). (E) Zoom-in confocal images presenting the selected A′ and A″ regions (white squares) within the hCOs at weeks 4 to 6 as those are originally depicted in (D) (scale bar, 100 μm).

Article Snippet: In the setup, the microscope was equipped with high- power light- emitting diode (LED) (nominal wavelength: 470 nm, Thorlabs, Newton, NJ, catalog no. M470LP- C2) as a fluorescence excitation source and a dual- tap complementary metal- oxide semiconductor (CMOS) FLIM camera (PCO.FLIM, Excelitas Technologies Corp., Waltham, MA) as an imaging sensor.

Techniques: Control, Cell Culture, Fluorescence, Staining, Clinical Proteomics, Membrane

Journal: Science advances

Article Title: Shaping early neural development by timed elevated tissue oxygen tension: Insights from multiomic analysis on human cerebral organoids.

doi: 10.1126/sciadv.ado1164

Figure Lengend Snippet: Fig. 3. Intra-organoid oxygen tension, structures, and cell compositions of hypoxia-treated hCOs. (A) Bright-field images of the hypoxia-treated hCOs cultured in the hydrogel matrix at different time points (scale bar, 400 μm), and fluorescence images of the hypoxia-treated hCOs stained with a plasma membrane stain, CellMask (green), and the embedded oxygen-sensing CPOx beads (red) (scale bar, 400 μm). (B) Comparison of the organoid size of the control and hypoxia-treated hCOs estimated from the captured bright-field images. Data are presented as box plots with all data points and mean (SD) (n = 10). (C) Comparison of the average oxygen tension values calculated based on the fluorescence lifetime measurement of CPOx beads embedded within the control and hypoxia-treated hCOs (intra-organoid oxygen tension). (D) Confocal images of hypoxia-treated hCO slices stained with TUBB3, SOX2, Nestin, and Ki-67 proteins and cell nuclei (DAPI) revealing the distribution of neurons and NSCs in the hCOs at weeks 5 and 6, respectively (scale bar, 400 μm). (E) Zoom-in confocal images presenting the selected A′ and A″ regions (white square) within the hypoxia-treated hCOs at weeks 5 and 6 (scale bar, 100 μm) as originally depicted in (D). (F) Comparison of VZ/SVZ and CP region thicknesses in the hCOs (control and hypoxia-treated ones) at weeks 5 and 6. The thicknesses are analyzed from the captured fluorescence images (n = 4). (G) Comparison of TUBB3+, SOX2+, and Ki-67+ cell numbers in the hCOs at weeks 5 and 6. The numbers are enumerated from the captured fluorescence images. One-way analysis of variance (ANOVA) with Tukey’s test is performed for statistical analysis. Data are presented as box plots and mean (SD) with all data points (n = 4). All the quantitative data are shown in table S3.

Article Snippet: In the setup, the microscope was equipped with high- power light- emitting diode (LED) (nominal wavelength: 470 nm, Thorlabs, Newton, NJ, catalog no. M470LP- C2) as a fluorescence excitation source and a dual- tap complementary metal- oxide semiconductor (CMOS) FLIM camera (PCO.FLIM, Excelitas Technologies Corp., Waltham, MA) as an imaging sensor.

Techniques: Cell Culture, Fluorescence, Staining, Clinical Proteomics, Membrane, Comparison, Control

Journal: Science advances

Article Title: Shaping early neural development by timed elevated tissue oxygen tension: Insights from multiomic analysis on human cerebral organoids.

doi: 10.1126/sciadv.ado1164

Figure Lengend Snippet: Fig. 5. Intra-organoid oxygen tension, structures, and cell compositions of shLacZ and NGB knockdown (shNGB) hCOs. (A) Bright-field images of the shLacZ and shNGB hCOs cultured in the hydrogel matrix at different time points (scale bar, 400 μm), and fluorescence images of the shLacZ and shNGB hCOs stained with CellMask (green) and the embedded oxygen-sensing CPOx beads (red) (scale bar, 400 μm). (B) Organoid sizes of the shLacZ and shNGB hCOs estimated from the captured bright- field images. Data are presented as box plots and mean (SD) with all data points. One-way ANOVA with Tukey’s test is performed for statistical analysis (n = 10). (C) Com- parison of the average oxygen tension values calculated based on the fluorescence lifetime measurement of CPOx beads embedded within the control, shLacZ, and shNGB hCOs (intra-organoid oxygen tension) at weeks 4 to 6. The data are expressed as mean (SD), and one-way ANOVA with Tukey’s test is performed for statistical analysis. The quantitative data are shown in table S4. (D) Confocal images of shLacZ and shNGB hCOs slices stained with TUBB3, SOX2, Nestin, and Ki-67 proteins and cell nuclei (DAPI), revealing the distribution of neurons and NSCs in the hCOs at weeks 4 to 6 (scale bar, 400 μm).

Article Snippet: In the setup, the microscope was equipped with high- power light- emitting diode (LED) (nominal wavelength: 470 nm, Thorlabs, Newton, NJ, catalog no. M470LP- C2) as a fluorescence excitation source and a dual- tap complementary metal- oxide semiconductor (CMOS) FLIM camera (PCO.FLIM, Excelitas Technologies Corp., Waltham, MA) as an imaging sensor.

Techniques: Knockdown, Cell Culture, Fluorescence, Staining, Control